İran’ın Kuzeydoğusundaki Çevre Suları Örneklerinden Nontüberküloz Mikobakterilerin İzolasyonu ve Tanımlanması

Houshyar AKBARI; Hosna ZARE; Kiarash GHAZVINI

doi:10.4274/mjima.galenos.2019.2019.26

İran’ın Kuzeydoğusundaki Çevre Suları Örneklerinden Nontüberküloz Mikobakterilerin İzolasyonu ve Tanımlanması

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ÖZGÜN MAKALE

İran’ın Kuzeydoğusundaki Çevre Suları Örneklerinden Nontüberküloz Mikobakterilerin İzolasyonu ve Tanımlanması

Mediterr J Infect Microb Antimicrob 2019;8:26-26 DOI: 10.4274/mjima.galenos.2019.2019.26

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İzin

Öz

Giriş: Çevresel insan enfeksiyonu kaynağı olarak son zamanlarda giderek artan düzeyde dikkat çeken ve toplamda 150’den fazla türü olan nontüberküloz mikobakterilere (NTM) verilen önem artmaktadır. Bu çalışmanın amacı, İran’ın kuzeydoğusunda çevresel NTM türlerini izole etmek ve tanımlamaktı.

Gereç ve Yöntem: Razavi Horasan eyaletinin 73 şehrindeki içme suyu dağıtım ağları ve su kaynaklarından toplanan 344 su örneği kültür yoluyla incelendi. Nontüberküloz mikobakteriler tanımlaması ayrıca hsp65 geninin polimeraz zincir reaksiyonu amplifikasyonu, sekanslama ve veri analizi yoluyla da yapıldı. İstatistiksel analiz, SPSS 16.0 kullanılarak Pearson korelasyon katsayısı testi ve iki örnek t-testi ile yapıldı. istatistiksel anlamlılık seviyesi 0,05 olarak belirlendi.

Bulgular: Nontüberküloz mikobakteriler, kültürde su örneklerinin %10,46’sında (36/244) tespit edilmiştir. En yaygın NTM türü Mycobacterium gordonae (13/36) olarak belirlenmiştir. Diğer saptanan türler M. mucogenicum, M. senegalense, M. gadium, M. genavense, M. simiae, M. frederiksbergense, M. flouranthenivorans, M. neoaurum, ve M. pallens’di. Ayrıca, suda NTM varlığı ile nitrat seviyesi, türbidite, pH ve su kaynağının yaşı arasında istatistiksel olarak anlamlı bir korelasyon gözlendi (p<0,001). Nontüberküloz mikobakteriler varlığı ile su kaynağının derinliği arasındaki ilişki istatistiksel olarak anlamlı bulunmadı (p>0,05).

Sonuç: Bu bölgede çevre sularında NTM kontaminasyonunun çok yüksek olmadığı sonucuna ulaşılmıştır, ancak suların kontaminasyon kaynaklarını ve bu türlerin kökenlerini araştırmak için ek çalışmalar yapılması gereklidir.

Anahtar Kelimeler: Nontüberküloz mikobakteri, çevre, su kaynakları, İran

Kaynakça

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