Öz
Giriş: Dünya çapında su ürünleri yetiştiriciliğinin büyümesi, süreğen enfeksiyonlara ve antibiyotiğe dirençli bakterilerin (ARB) ortaya çıkmasına neden olmuştur. Bu çalışma, minimum inhibitör konsantrasyon (MIC) verilerine dayanan kümeleme algoritmalarını kullanarak Hindistan’daki Doğu Kolkata sulak alanında ve Kentsel Kolkata’da kültürlenen sazanların otokton bakteriyel florasındaki antibiyotik duyarlılığının benzerliğini ve bağıntısını tanımlamaktadır.
Gereç ve Yöntem: Sazanlardan ve çevrelerinden seçici ortamda izole edilen hareketli Aeromonas spp. ve Escherichia coli (her biri 50) agar-disk difüzyon deneyi kullanılarak oksitetrasikline ve kloramfenikole duyarlılık açısından test edildi. Bu iki antibiyotiğin MIC’leri, agar seyreltme yöntemi kullanılarak belirlenmiş ve BioNumerics 7.6 yazılım paketi kullanılarak kümelenmiştir.
Bulgular: Oksitetrasiklin ve kloramfenikolün MIC’leri sırasıyla 0,39-50 ug/ml ve 1,56 ila >100 ug/ml arasında değişmiştir. Hareketli aeromonadların dendrogram tabanlı küme analizi, ana kümeler altında >5 alt grup elde edildiğinden, nispeten yüksek iç homojenlik göstermiştir. Escherichia coli ayrıca yüksek iç homojenlik göstermiştir. Bir grup olarak hareketli aeromonadların dendrogram tabanlı gelişmiş düğüm küme analizi, daha fazla sayıda küme vermiştir.
Sonuç: Antibiyotik kullanımı öyküsü olmayan bir kültür balıkçılığı ortamından izole edilen hareketli aeromonadlar ve E. coli’de saptanan değişken duyarlılıklar, sazanların ARB’lerle ev ve hastane atıklarından olası kontaminasyonunu düşündürmüştür. Bununla birlikte, bu ortamdan izole edilen E. coli suşları, ciddi bir endişe nedeni olan antibiyotik duyarlılığında yüksek heterojenite sergilemiştir.
Anahtar Kelimeler: Hareketli aeromonadlar, Escherichia coli, antibiyotikler, minimum inhibitör konsantrasyon, küme analizi
Kaynakça
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