Öz
Giriş: Karbapenem dirençli Acinetobacter baumannii ile ilişkili hastane enfeksiyonları, yoğun bakım ünitelerinde yatan kritik hastalarda önemli bir sorun olarak kabul edilmektedir. Bu çok ilaca dirençli mikroorganizmanın neden olduğu enfeksiyonları tedavi etmek için yeni antimikrobiyal ajanların olmaması, bu tür enfeksiyonlarla mücadele etmek için yeni stratejilerin araştırılmasını teşvik etmektedir. Bu nedenle, bu çalışmanın amacı, iki yoğun bakım ünitesinden izole edilen karbapenem dirençli Acinetobacter baumannii’ye karşı Lavandula mairei Humbert esansiyel yağının antibakteriyel etkinliğini ilk kez değerlendirmektir.
Gereç ve Yöntem: Karbapenemaz kodlayan genler, polimeraz zincir reaksiyonu ve dizileme yöntemleri ile tespit edildi. Lavandula mairei esansiyel yağının kimyasal bileşimi gaz kromatografisi-kütle spektrometrisi analizi ile belirlendi ve antibakteriyel aktivitesi disk difüzyon ve et suyu mikrodilüsyon yöntemleri ile değerlendirildi.
Bulgular: Seçilen suşlarda OXA-23 ve NDM-1 varyantları ortaya çıktı. Lavandula mairei esansiyel yağında, karvakrolün en baskın olduğu (%77,32) 26 bileşik tespit edildi. Test edilen tüm karbapenem dirençli Acinetobacter baumannii suşları, 28,67±1,15 mm ila 40±2,00 mm arasında değişen inhibisyon bölgelerinin çapları ile yağa karşı geniş bir duyarlılık gösterdi ve minimum inhibitör ve bakterisidal konsantrasyon değerlerinin benzerliği ile (0,39 ila 3,125 µL/ml) kanıtlanmış bir bakterisidal etki gösterdi.
Sonuç: Bu bulgular, doğal antibakteriyel ajanların kaynağı olarak Lavandula mairei esansiyel yağının gelecekteki kullanımı için çok umut verici olmaya devam etmektedir.
Anahtar Kelimeler: Antibakteriyel aktivite, karbapenem dirençli Acinetobacter baumannii, esansiyel yağlar, Lavandula mairei Humbert, hastane enfeksiyonları
Kaynakça
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