Öz
Giriş: Son yıllarda patojenik bakterileri yok etmek için nanopartiküllerin (NP) kullanımı artmaktadır. Nanopartikülleri sentezlemek için biyolojik ve toksik olmayan yöntemlerin geliştirilmesi, nanoteknolojide bu NP’lerin sağlık ve tıbbi amaçlar için kullanımına izin vermek için önemli bir konudur. Bu çalışma, bakteri kültür ortamı kullanılarak gümüş NP’leri (AgNP) sentezlemeyi ve ardından bu NP’lerin Acinetobacter baumannii’ye karşı antibakteriyel aktivitesini değerlendirmeyi amaçladı.
Gereç ve Yöntem: Biyouyumlu AgNP’lerin sentezi beyin kalp infüzyonu (BHI) kültür ortamı ile gerçekleştirildi. Sentezlenen NP’ler, ultraviyole-görünür spektroskopisi kullanılarak gözlendi ve Fourier transform kızılötesi spektroskopisi (FTIR) ile karakterize edildi. Gümüş NP’lerin morfolojisini incelemek için taramalı elektron mikroskobu ve transmisyon elektron mikroskobu (TEM) kullanıldı. Gümüş NP’lerin antibakteriyel etkisini ölçmek için minimum inhibitör konsantrasyon (MIC) belirlendi. Son olarak sentezlenen AgNP’lerin antibiyofilm aktiviteleri değerlendirildi.
Sonuçlar: Ultraviyole-görünür spektroskopisi, sentezlenen NP’lerin en yüksek absorpsiyonunun 420 nm’de olduğunu gösterdi. Taramalı elektron mikroskobu ve TEM sonuçları, parçacıkların morfolojisinin küresel ve düzensiz olduğunu ve ortalama çaplarının 26 nm olduğunu ortaya çıkardı. Ek olarak, FTIR sonuçları, NP’lerin indirgeyici, örtücü ve stabilize edici ajanlarının proteinler olduğunu doğruladı. Gümüş NP’lerin MIC değeri 1,56 ila 12,5 µg/ml aralığındaydı ve 2 MIC’de, NPS’nin biyofilm oluşumunu inhibe edici aktivitesi arttı.
Sonuç: Stabil AgNP’lerin sentezlenmesi için BHI ortamı başarıyla uygulandı ve bu NP’ler A. baumannii’ye karşı iyi antibakteriyel ve antibiyofilm aktivitesi gösterdi.
Anahtar Kelimeler: Gümüş nanoparçacıklar, sentez, antibiyofilm, antibakteriyel, Acinetobacter baumannii
Kaynakça
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