Öz
Giriş: Non-fermentatif bir bakteri olan Stenotrophomonas maltophilia, ağırlıklı olarak bağışıklığı baskılanmış bireylerde ve uzun süreli, geniş spektrumlu antimikrobiyal ajanlar alanlarda fırsatçı enfeksiyonlara neden olur. S. maltophilia, mevcut antimikrobiyal ajanların çoğuna doğal olarak dirençlidir. Trimetoprim-sulfametoksazol (TMP/SMX), seftazidim ve levofloksasin (LVX) enfeksiyonların tedavisinde tercih edilen ilaçlardır. Bu çalışmanın amacı, S. maltophilia izolatlarında plazmit aracılı kinolon direnç belirleyicileri olan QnrA, QnrB, QnrC,QnrD, QnrS ve OqxAB genlerinin varlığını ortaya koymaktır.
Gereç ve Yöntem: Çalışmada çeşitli klinik örneklerden toplanan 122 S. maltophilia klinik izolatı test edilmiştir. Trimetoprim-sulfametoksazol ve LVX duyarlılığı disk difüzyon yöntemi ile, seftazidim (CAZ) minimum inhibitör konsatrasyon (MİK) değeri ise gradient difüzyon testi ile belirlendi. Qnr (A, B, C, S, D) determinantları ve OqxAB varlığı multipleks polimeraz zincir reaksiyonu ile araştırıldı. İlk olarak OqxA ve OqxA pozitif izolatlarda OqxB varlığı araştırıldı.
Bulgular: S. maltophilia izolatlarının beşi (%4) TMP/SMX’ye dirençliydi. Levofloksasin ve CAZ direnci sırasıyla %6,5 ve %56,5 olarak belirlendi. İki izolatta QnrS tespit edildi. Üç izolatta OqxA geninin varlığı tespit edildi ancak üç izolatta da OqxB tespit edilmedi. Bu üç pozitif izolattan birinin de daha önce QnrS pozitif olduğu bulunmuştur.
Sonuç: Çalışmamızda S. maltophilia izolatlarında ikisinde QnrS, üçünde OqxA geninde tespit edildi.
Anahtar Kelimeler: Plazmit aracılı direnç, Qnr, OqxAB, S. maltophilia
Kaynakça
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