Öz
Giriş: Sepsis, kan dolaşımını istila eden mikrobiyal patojenlere bağlı olarak ortaya çıkan konakçı sistemik enflamatuvar yanıt sendromudur. Hızlı ve doğru tanı, sepsis tedavisinde komplikasyonlar oluşmadan önce anahtar rol oynar. Bu nedenle, bu çalışmada fenotipik yöntem ve polimeraz zincir reaksiyonu (PCR) tekniği kullanılarak kan kültüründen sepsisin bakteriyel ajanlarının belirlenmesi amaçlanmıştır.
Gereç ve Yöntem: İmam Rıza Hastanesi’ndeki sepsis şüphesi olan hastalardan toplam 287 adet kan kültürü şişesi alınarak tıp fakültesi mikrobiyoloji laboratuvarına nakledildi. İzolatlar, Microgen Kit ve API 20 E kiti ile tanımlandı. Bakteriyel izolatların moleküler tespiti için 16S rDNA geninin PCR sekans analizi uygulandı.
Bulgular: İki yüz seksen yedi şüpheli sepsis örneğinden 231’i negatif, 56’sı pozitif olarak belirlendi. Pozitif numunelerin beşinde kültürde üreme olmamasına rağmen PCR ile pozitiflik saptandı. Elli altı izolattan 16’sı (%28,57) Gram-olumsuz bakteri, 40’ı (%71,42) Gram-olumlu bakteri idi.
Sonuç: Bu çalışmada, PCR tabanlı 16S rDNA geni yöntemi diğer bakteri tespit yöntemlerine kıyasla yüksek duyarlılığa sahipti. Genel olarak, hızlı örnekleme ve hızlı ve doğru teşhis, gereksiz antibiyotik kullanımını azaltır.
Anahtar Kelimeler: Kan kültürü, bakteriyel izolatlar, fenotipik tanımlama, genotipik tanımlama, polimeraz zincir reaksiyonu
Kaynakça
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